Part:BBa_K2446036:Design

SV40_UAS promoter

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part is aseembled by the specfic DNA binding sites and Sv40 promoter through Biobrick assembly. We inset the DNA binding sites behind the Sv40 promoter. Then the DNA binding domain with KRAB can repress the expression of this promoter. The repression intensity can be adjusted by change the repeats of DNA binding sites.

Source

Assembled by Sv40 promoter (BBa_K2446052) and 4*GAL4 binding sites from UAS-Gal4 Promoter MammoBlock(BBa_K511003 MIT2011) in pSB1C3 through Biobrick assembly.

References

[1] L. Morsut et al., Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Cell 164, 780--791 (2016).

[2] R. Witzgall, E. O'Leary, A. Leaf, D. Onaldi, J. V. Bonventre, The Krüppel-associated box-A (KRAB-A) domain of zinc finger proteins mediates transcriptional repression. Proceedings of the National Academy of Sciences 91, 4514-4518 (1994).

[3]2011_MIT http://2011.igem.org/Team:MIT